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| Title: | Monoclonal antibodies to human hemoglobin S and cell lines for the production thereof |
| Inventors: | Jensen, Ronald H.; Livermore, CA, USA Vanderlaan, Martin; San Ramon, CA, USA Bigbee, William L.; Livermore, CA, USA Stanker, Larry H.; Livermore, CA, USA Branscomb, Elbert W.; Walnut Creek, CA, USA Grabske, Robert J.; Berkeley, CA, USA |
| Summary: | This invention describes monoclonal antibodies specific to the sickle cell hemoglobin and methods of their production and use in the detection of sickle cell traits and in the identification of somatic cell mutations. Described herein are methods of producing monoclonal antibodies which are capable of distinguishing between hemoglobin S and hemoglobin A, such that these two types of hemoglobin differ from each other by a single amino acid residue. Further disclosed are techniques by which the antibodies produced according to this method may be used as immunofluorescent markers to enumerate circulating red blood cells which have the property of altered expression of the hemoglobin gene due to somatic mutation in stem cells. Claims of the invention include a high degree of specificity and a greater affinity of the antibodies for hemoglobin S. Therapeutic and experimental applications are included as an assay for in vivo cellular somatic mutations in humans and for the labeling of human red blood cells for detection by flow cytometry of hemoglobin genotypes. |
| Abstract: | The present invention provides monoclonal antibodies specific to and distinguish between hemoglobin S and hemoglobin A and methods for their production and use. These antibodies are capable of distinguishing between two hemoglobin types which differ from each other by only a single amino acid residue. The antibodies produced according to the present method are useful as immunofluorescent markers to enumerate circulating red blood cells which have the property of altered expression of the hemoglobin gene due to somatic mutation in stem cells. Such a measurement is contemplated as an assay for in vivo cellular somatic mutations in humans. Since the monoclonal antibodies produced in accordance with the instant invention exhibit a high degree of specificity to and greater affinity for hemoglobin S, they are suitable for labeling human red blood cells for flow cytometric detection of hemoglobin genotype. |
| US Patent Website: | Click Here for Full Text of Patent |
| Title Number: | US4752583 |
| Application Number: | US1984000676162 |
| Date Filed: | 29/11/1984 |
| Date Published: | 21/06/1988 |
| Assignee: | The USA as represented by the United States Department of Energy, Washington, DC, USA |