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Stem Cell Related Patent Number US5525708
| Title: | Covalent dimer of kit ligand | | Inventors: | Nocka, Karl H.; Harvard, MA, USA
Lobell, Robert B.; Watertown, MA, USA | | Summary: | This invention describes a method of preparing a modified form of kit ligand, wherein the protein has been stabilized by an intermolecular covalent linkage. The biologically active, interchain disulfide-linked dimer of kit ligand consists of 2 monomers, each one having the same amino acid sequence, with the covalent dimer being free of such monomers and inactive dimers of kit ligand. | | Abstract: | A modified form of KL, the ligand for the c-Kit proto-oncogene, has been prepared wherein the protein is stabilized by an intermolecular covalent linkage. The protein can be prepared by expression of a recombinant protein which is dissolved in denaturant and refolded under conditions resulting in a disulfide linked dimer. Examples demonstrate the purification and characterization of this disulfide-linked cysteine dimer kit ligand (KL-CD) which contains at least one intermolecular disulfide bond and has at least ten-fold greater activity in promoting cell proliferation than native, non-covalently linked KL, as measured in in vitro assays. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US5525708 | | Application Number: | US1994000220379 | | Date Filed: | 28/03/1994 | | Date Published: | 11/06/1996 | | Assignee: | CytoMed, Inc., Cambridge, MA, USA |
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