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| Title: | Method for the preparation of in vitro-derived human neutrophil precursor cells |
| Inventors: | Bender, James G.; Lindenhurst, IL, USA Maples, Phillip B.; Waukegan, IL, USA Smith, Stephen; Arlington Heights, IL, USA Unverzagt, Kristen L.; Palatine, IL, USA Van Epps, Dennis E.; Cary, IL, USA |
| Summary: | Described herein are compositions containing human neutrophil precursor cells with a high content of myeloblasts and pro-myelocytes. The invention relates to such a composition in which at least 16% of the cells are human myeloblasts and promyelocytes derived from human neutrophil progenitor cells that were obtained from peripheral blood, bone marrow or cord blood. Further disclosed are methods of preparing such compositions from CD34+ hematopoietic progenitor cells which are cultured in the presence of at least one hematopoietic growth factor to obtain a suspension of proliferating human neutrophil precursor cells. Therapeutic applications are included for the treatment of genetic or acquired neutropenia. |
| Abstract: | A composition of human neutrophil precursor cells is disclosed wherein at least 16% of the cells are human myeloblasts and promyelocytes. The myeloblasts and promyelocytes are derived from human neutrophil progenitor cells that were obtained from peripheral blood, bone marrow or cord blood. The neutrophil precursor cells contain less than 5% colony forming units. Also disclosed are human neutrophil precursor cells made up of about 16% CD15+CD11b- cells and less than 5% colony forming units and methods of preparing these compositions. |
| US Patent Website: | Click Here for Full Text of Patent |
| Title Number: | US5700691 |
| Application Number: | US1996000707762 |
| Date Filed: | 13/09/1996 |
| Date Published: | 23/12/1997 |
| Assignee: | Baxter Healthcare Inc., Irvine, CA, USA |