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Stem Cell Related Patent Number US6153113
| Title: | Method for using ligands in particle separation | | Inventors: | Goodrich, Raymond P.; Denver, CO, USA
Green, Todd Curtis; Lakewood, CO, USA | | Summary: | This invention describes a system and method for separating particles having different sedimentation velocities. The system includes a container containing a binding substance including first particles and ligands that are attached to the first particles. Means are described by which the binding substance is mixed with a liquid carrying at least second and third particles, during which the ligands bind the first and second particles together to form groups of bound particles, which are later separated from the third particles in a fluid chamber configured to be mounted on a centrifuge rotor. Also disclosed are methods for forming a saturated fluidized bed of particles to retain the particle groups in the fluid chamber, as are means of removing at least some of the first particles after the binding substance is mixed with the liquid carrying second and third particles. Applications and uses are also described in the isolation of specific blood cells. | | Abstract: | A system and method are disclosed for separating particles having different sedimentation velocities. The system includes a container containing a binding substance including first particles and ligands attached to the first particles. When the binding substance is mixed with a liquid carrying at least second and third particles, the ligands bind the first and second particles together to form groups of bound particles. The groups of bound particles are separated from the third particles in a fluid chamber configured to be mounted on a centrifuge rotor. One of the disclosed methods includes forming a saturated fluidized bed of particles to retain the particles groups in the fluid chamber. Another of the disclosed methods includes removing at least some of the first particles after the binding substance is mixed with the liquid carrying second and third particles. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US6153113 | | Application Number: | US1999000253696 | | Date Filed: | 22/02/1999 | | Date Published: | 28/11/2000 | | Assignee: | Cobe Laboratories, Inc., Lakewood, CO, USA |
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