Methods comprising apoptosis inhibitors for the generation of transgenic pigs |
 |
|
|
||||||||||||||||||||||||||||||||||||||||||
| Title: | Method of genetically modifying very primitive quiescent human hematopoietic stem cells |
| Inventors: | Chatterjee, Saswati; Sierra Madre, CA, USA Wong, Jr., Kamehameha K.; Sierra Madre, CA, USA Wong, Christie; Pasadena, CA, USA Fisher-Adams, Grace; La Canada , CA, USA |
| Summary: | Described herein is a method for stably transferring DNA into multipotent hematopoietic stem cells, thereby transducing extremely primitive hematopoietic stem cells with high efficiency. Further disclosed is the use of an adeno-associated vector for such transduction, said vector being capable of stably transforming highly primitive CD34+++CD38= cells which reside in a quiescent state, thereby retaining to a larger extent the ability to repopulate the hematopoietic system. Methods of DNA integration are provided, as are therapeutic applications in gene therapy for inherited metabolic diseases, viral infections, and cancer. |
| Abstract: | This invention relates to a method for transducing extremely primitive hematopoietic stem cells with high efficiency, using an adeno-associated vector. This vector stably transforms highly primitive CD34+++CD38= cells which reside in a quiescent state and retain to a larger extent the ability to repopulate the hematopoietic system. |
| US Patent Website: | Click Here for Full Text of Patent |
| Title Number: | US6919209 |
| Application Number: | US1999000453801 |
| Date Filed: | 03/12/1999 |
| Date Published: | 19/07/2005 |
| Assignee: | City of Hope, Duarte, CA, USA |