| Title: | Directed differentiation of human embryonic cells |
| Inventors: | Benvenisty, Nissim; Jerusalem, Israel |
| Summary: | This invention involves novel methods for mapping and directing a pathway of differentiation of a population of human embryonic stem cells into neuronal cells. The chemically dissociated embyoid bodies are exposed to exogenous factors which enrich the culture for the specific cell type that is desired. Methods of aggregation of the hES to form the embryoid bodies are also described, as are methods of dissociating the embryoid bodies into single cells. |
| Abstract: | Methods are described for mapping a pathway of differentiation of a population of embryonic cells which includes exposing the cells to an exogenous factor and measuring gene expression products that are characteristic of a particular cell type or lineage. Directing differentiation of human embryonic cells relies on dissociated embryoid bodies which are then exposed to one or more exogenous factors to enrich a culture for a particular cell type. The differentiated cells may be used for treating a medical condition in a human. Kits for determining differentiation pathways and screening exogenous factors for their utility in differentiation are provided. |
| US Patent Website: | Click Here for Full Text of Patent |
| Title Number: | US7045353 |
| Application Number: | US2001000918702 |
| Date Filed: | 31/07/2001 |
| Date Published: | 16/05/2006 |
| Assignee: | Yissum Research Development Company of The Hebrew University of Jerusalem, Jerusalem, Israel |
